Specialized protein assay reagents and kits to estimate and measure specific post-translational modifications (PTM) or functional (enzymatic) classes of proteins in biological samples, including glycoproteins, phosphoproteins, and proteases.
Hydrogen Peroxide Chemiluminescent Detection Kit is a higher throughput assay for the quantitative measurement of hydrogen peroxide in culture supernatants and cell lysates from any species.
The ADP-Glo™ Max Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure ATPase or kinase activity by quantifying the amount of ADP produced in a reaction.
BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides found in extracellular amyloid plaques of Alzheimer's disease (AD).
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Tobacco Etch Virus protease (TEV protease) is a highly sequence specific cysteine protease. It has a strict 7 amino acid cleavage recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln (Gly/Ser). The high specificity makes this protease excellent for the removal of affinity-tags from purified recombinant proteins. The kit contains a TEV protease substrate that is labeled with fluorophore FAM and a quencher. Proteolytic activity of TEV protease cleaves the substrate and releases the FAM, resulting in the production of bright fluorescence which can be measured using a fluorescence reader at ex/em of 490 nM/520 nm. TEV protease activity then can be calculated in accordance with the fluorescence intensity. Purified TEV protease is included in the kit as a positive control.
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The kit essentially generates spheroplasts of yeast cells for subsequent analysis. It is optimized for S. cerevisiae and C. albicans. The kit can also be used for any fungus species that is susceptible to yeast lytic enzyme digestion.
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Tr-Fret Assay, Not Antibody-Based, Screen Any Kinases, Phosphatases, And Phosphodiesterases , Sufficient To Generate 800 Data Points In A Standard 384 Well Format
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MW 742.95 g/mol, Purity >93%. Fluorescent triplet probe used for the measurement of rotational diffusion of protein in solution and in membranes, including myosin and pyruvate dehydrogenase. Widely used in a flow cytometry test to detect hereditary spherocytosis (HS).
MW 304.43 g/mol. Potent, selective inhibitor phosphodiesterase PDE-11 inhibitor (IC₅₀ = 0.28 μM). IC₅₀s for PDE1-10 are greater than 100 μM. Significantly elevates cAMP levels and cortisol production in H295R human adenocarcinoma cells.
Tr-Fret Assay, Not Antibody-Based, Screen Any Kinases, Phosphatases, And Phosphodiesterases , Sufficient To Generate 8,000 Data Points In A Standard 384 Well Format
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MW 304.43 g/mol. Potent, selective inhibitor phosphodiesterase PDE-11 inhibitor (IC₅₀ = 0.28 μM). IC₅₀s for PDE1-10 are greater than 100 μM. Significantly elevates cAMP levels and cortisol production in H295R human adenocarcinoma cells.