Class-Specific Protein Assay Kits
Abnova™ Hydrogen Peroxide Chemiluminescent Detection Kit
Hydrogen Peroxide Chemiluminescent Detection Kit is a higher throughput assay for the quantitative measurement of hydrogen peroxide in culture supernatants and cell lysates from any species.
Abnova™ Hydrogen Peroxide Colorimetric Detection Kit
Hydrogen Peroxide Colorimetric Detection Kit is a higher throughput assay for the quantitative measurement of hydrogen peroxide in culture supernatants, serum, plasma, and urine from any species.
Promega Proteasome-Glo™ 3-Substrate System
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in a purified enzyme preparation.
Dojindo Molecular Technologies Inc SPiDER-gal (20UG x3) is a fluorogenic beta-galactosidase detection probe applicable for living cells and tissues.
The gene of β -galactosidase from E. coli is widely used as a reporter gene assay marker. Although X-gal is a well known reagent to detect β -galactosidase in cell or tissue samples, the assay using these reagents require to fix cells or tissues due to the poor cell-permeability. In addition, the assay using fluorescence reagents can not clearly differentiate β -galactosidase - expressed cells or regions in its current development. SPiDER-Gal ideally possesses cell-permeability and the ability to retain in the intracellular region. With the enzymatic reaction, SPiDER-Gal immediately forms a quinone methide that acts as electrophile when proteins containing nucleophilic functional groups are nearby the molecules. When the probe undergoes the reaction with a protein, the conjugates become fluorescent compounds. SPiDER-βGal allows a single-cell analysis because it self-immobilizes to the intracellular proteins.
Bioassay Systems QuantiChrom™ Lactate Dehydrogenase Kit. For quantitative determination of lactate dehydrogenase LDH activity and screen/evaluation of LDH modulators.
For quantitative determination of lactate dehydrogenase LDH activity and screen/evaluation of LDH modulators. Key Features: High sensitivity and wide linear range. Use 3 µL serum or plasma sample. The detection limit is 2 U/L, linear up to 200 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of LDH activity within 30 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD565nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2 U/L.
AURORA BIOLABS LLC TEV Protease Activity Assay, 96 rxn
Tobacco Etch Virus protease (TEV protease) is a highly sequence specific cysteine protease. It has a strict 7 amino acid cleavage recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln ↓ (Gly/Ser). The high specificity makes this protease excellent for the removal of affinity-tags from purified recombinant proteins. The kit contains a TEV protease substrate that is labeled with fluorophore FAM and a quencher. Proteolytic activity of TEV protease cleaves the substrate and releases the FAM, resulting in the production of bright fluorescence which can be measured using a fluorescence reader at ex/em of 490 nM/520 nm. TEV protease activity then can be calculated in accordance with the fluorescence intensity. Purified TEV protease is included in the kit as a positive control.
TRIBIOSCIENCE INC Tryptase Activity Assay, 100 Tests
The Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for the evaluation of tryptase activity in cell lysates, supernatants, or for inhibitor screening.
Bio Rad Laboratories BIORAD DC PROTEIN ASSAY KIT II
Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, BSA standard; 450 assays
Cell Biolabs Inc Beta-Hexosaminidase Assay Kit, 100 assays
Hexosaminidase is an enzyme that hydrolyzes terminal N-acetyl-D-hexosamine residues from N-acetyl-ß-D-hexosaminidines. The enzyme is typically found as a dimer of the alpha and beta subunits. Cell Biolabs' Beta Hexosaminidase Assay Kit quantifies the activity of the beta subunit in a convenient 96-well microplate format. Each kit provides sufficient reagents for 100 assays including standards and unknown samples.
AURORA BIOLABS LLC TEV PROTEASE HIS-TAG. 200 μg
Tobacco Etch Virus nuclear-inclusion-a endopeptidase or TEV is highly sequence specific cysteine protease. It has a strict 7 amino acid cleavage recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln ↓ (Gly/Ser). The high specificity makes this protease excellent for the removal of affinity-tags from purified recombinant proteins. Purified TEV was expressed in E.coli and is shipped in 4°C packaging to preserve activity. TEV cleavage is best performed at room temperature in a P.H range of 4 – 9